Cell Based Assays
Promega NUAK2 Kinase Enzyme System, 1mg
Recombinant full-length human NUAK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NUAK2 or SNF1/AMP kinase-related kinase (SNARK) is a member of the NUAK family of SNF1-like kinase 2.
Promega ADP-Glo™ Kinase Assay + CDK6/CyclinD3 Kinase Enzyme System
Recombinant full-length human CDK6 and CyclinD3 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal His tag on both proteins.
Promega ADP-Glo™ Kinase Assay + DYRK2 Kinase Enzyme System
Recombinant full-length human DYRK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + CK1 gamma 1 Kinase Enzyme System
Recombinant human CK1 gamma 1 (21-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + TAK1-TAB1 Kinase Enzyme System
Recombinant human proteins TAK1 (1-303) and TAB1 (437-end), linked by a small peptide (DFGGGGG), were expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + MARK1 Kinase Enzyme System
Recombinant full-length human MARK1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MARK1 is a member of the MARK family and is a serine/threonine-protein kinase that plays a key role in signal transduction.
Promega ADP-Glo™ Kinase Assay + ERK2 Kinase Enzyme System
Recombinant full-length human ERK2 was expressed by E. coli cells using an N-terminal GST tag and activated by MEK1 in vitro. ERK2 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs).
Promega ADP-Glo™ Kinase Assay + RET (V804L) Kinase Enzyme System
Recombinant human RET (V804L) (amino acids 658-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Promega ADP-Glo™ Kinase Assay + CDK5/p35 Kinase Enzyme System
Recombinant full-length human CDK5 and human p35 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CDK5 is a member of the Cyclin-Dependent Kinase family that is most abundant in the mammalian brain.
Promega TRKB Kinase Enzyme System, 1mg
Recombinant human TRKB (455-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TRKB is a member of the neurotrophic tyrosine receptor kinase (NTRK) family.
Promega ADP-Glo™ Kinase Assay + PYK2 Kinase Enzyme System
Recombinant human PYK2 (360-690) was expressed by baculovirus in Sf9 cells using an N-terminal His tag. PYK2 (also known as FAK2/RAFTK) is a member of the focal adhesion PTK family.
Promega ADP-Glo™ Kinase Assay + FMS Kinase Enzyme System
Recombinant human FMS (539-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. FMS is a proto-oncogene that encodes the tyrosine kinase transmembrane receptor for colony stimulating factor 1 receptor (CSF1R).
Promega MINK1 Kinase Enzyme System, 1mg
Recombinant human MINK1 (1-320) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. MINK1 is a member of the germinal center family of kinases that are homologous to the Ste20 family and regulate a wide variety of cellular processes.
Promega ASK1 Kinase Enzyme System, 1mg
Recombinant human ASK1 (649-946) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1) and MKK6. Overexpression of ASK1 induces apoptotic cell death.
Promega HER4 Kinase Enzyme System, 1mg
Recombinant human HER4 (amino acids 682-993) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. HER4 or ERBB4 is a transmembrane receptor tyrosine kinase that belongs to the epidermal growth factor receptor family.